PROTEOMICS

Mass spectrometry (MS) based proteomics has become an essential tool for biologists over the last decade. The ability of a mass spectrometer to identify thousands of proteins from a complex biological sample has revolutionized scientific experiments.

To fully understand the function of the proteome in health and disease, however, one must have the ability to accurately quantify proteins in many different types of biological samples. The invention of faster and higher resolution mass spectrometers has enabled the quantification of complex proteome dynamics. Heavy stable isotopes are routinely employed to generate precise and accurate quantitative proteomic data.

Peptides labelled with heavy stable isotopes share identical biochemical characteristics to “light” or unlabelled peptides except a difference in mass. Mixing heavy peptides with light peptides results in peptide pairs that co-elute into the mass spectrometer, which can easily distinguish between the peptides based on the mass difference.

Quantifying differences between proteomes subjected to different biological conditions or experiments can be achieved when using the heavy peptides as an internal standard or a control.

CIL offers a variety of stable isotope reagents for labelling any proteome for quantitative MS analysis. These stable isotope MS methods are bringing scientists closer to curing human diseases through quantitative biomarker analysis and quantitative proteomic analysis of animal models of disease.

- SILAC Kits & Reagents
- SILAM - Stable Isotope Labeling in Mammals
- PeptiQuant™ MRM Assay Kits are used to assess and track LC/MS performance of the LC/MS platform in a proteomic workflow in order to highlight any possible issues affecting quantitation.
- Heavylabeled ApoA-1 internal standard: Apolipoprotein A-1 (ApoA-1) is the primary protein component of high-density lipoprotein (HDL) in plasma and has a specific role in lipid metabolism.
- INLIGHT™ can be applied to complex N-linked glycome samples; a detailed protocol will be included demonstrating INLIGHT™ quantification of the N-linked glycome derived from plasma.

Buchem BV is proud distributor for CIL products in the Benelux.

Contact us for further information.